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Core practicals and practical skills

Pre-release article (written for this paper): Cities and the wildlife that lives in them

(1) More than half of the world's people now live in cities, and cities are growing faster than any other habitat on Earth. (2) For a long time, ecologists treated cities as biological deserts. (3) Recent studies show that many species not only survive in cities but change in response to them. (4) In several European cities, blackbirds sing at a higher pitch than blackbirds in nearby forests. Low-frequency traffic noise masks lower notes, and birds that sing higher are more likely to attract mates. (5) A small fish called the Atlantic killifish has evolved resistance to industrial pollutants in some heavily contaminated estuaries. Resistant populations carry mutations in genes coding for a receptor protein that normally triggers a toxic response to these chemicals. (6) Moths that live near bright street lights are, in some populations, less strongly attracted to light than moths from dark rural areas. (7) Critics warn that some reported differences may be due to phenotypic plasticity rather than evolution: individuals may simply respond to their surroundings during their lifetime, without any change in allele frequency. (8) To tell the difference, researchers raise animals from city and rural populations under identical conditions. If the differences remain, a genetic basis is likely. (9) Urban green spaces, such as parks, gardens and green roofs, can act as stepping stones between habitats, but small isolated populations may lose genetic diversity.
From Paper 3: General and Practical Principles in Biology
1(d)4 marks
Describe and explain the experiment in sentence 8 that distinguishes evolution from phenotypic plasticity.
From Paper 3: General and Practical Principles in Biology
2(a)4 marks
A student compared leaf surface area in sun and shade leaves of a tree (20 leaves each). Mean areas were 38 cm² (sun) and 54 cm² (shade). She calculated t = 4.6 with 38 degrees of freedom. The critical value at p = 0.05 is 2.02. (a) State the null hypothesis. (b) Interpret the result.
From Paper 3: General and Practical Principles in Biology
2(b)3 marks
Explain why the student should select leaves at random, and describe how she could do this.
From Paper 3: General and Practical Principles in Biology
3(a)5 marks
Describe how to prepare and stain a root tip squash to observe stages of mitosis.
From Paper 3: General and Practical Principles in Biology
3(c)3 marks
Give two hazards in this practical and how to reduce the risk of each.
From Paper 3: General and Practical Principles in Biology
4(a)4 marks
A student measured the heart rate of 10 volunteers before and after drinking caffeinated coffee. Describe how the investigation could be improved to give valid results.
From Paper 3: General and Practical Principles in Biology
4(b)3 marks
Give three ethical considerations for this investigation.
From Paper 3: General and Practical Principles in Biology
5(a)4 marks
Describe how you would use a dissected mammalian heart to compare the thickness of the left and right ventricle walls, and explain the difference you would expect.
From Paper 3: General and Practical Principles in Biology
5(b)4 marks
Describe how you would determine the water potential of potato tissue.
From Paper 3: General and Practical Principles in Biology
6(a)4 marks
Describe how you could investigate the effect of light intensity on the rate of photosynthesis using pondweed.
From Paper 3: General and Practical Principles in Biology
7(a)4 marks
Describe how to use a belt transect to investigate the change in plant species from the edge of a woodland into a field.
From Paper 3: General and Practical Principles in Biology
7(b)4 marks
Spearman's rank correlation between light intensity and percentage cover of a grass species along the transect was 0.71 for 10 quadrats (critical value at p = 0.05: 0.648). Interpret this result and explain why it does not prove that light controls the grass cover.
From Paper 3: General and Practical Principles in Biology
8*9 marks
Plan an investigation to compare the effectiveness of two antimicrobial substances (for example, two mouthwashes) on the growth of a harmless bacterium. Include the method, control variables, safety precautions, and how you would analyse the results.
From Paper 3: General and Practical Principles in Biology
94 marks
Clear zones around discs soaked in mouthwash A had diameters 14, 16, 15, 13 and 17 mm. Calculate the mean area of the clear zone (area = πr²), giving your answer to 3 significant figures.
From Paper 3: General and Practical Principles in Biology
103 marks
Explain why bacterial cultures in school laboratories are incubated at 25 °C rather than 37 °C.
From Paper 3: General and Practical Principles in Biology
116 marks
A student says: 'The bigger the clear zone, the better the mouthwash will be at preventing tooth decay.' Evaluate this statement.
Score 0 / 68 · 0 of 16 marked

This paper is written by Plasmo in the style of the Edexcel A-Level Biology exam. It is not a past paper and is not produced or endorsed by the exam board.

Core practicals and practical skills Exam Questions: Edexcel A-Level Biology | Plasmo